7DWR

Structure of Sulfolobus solfataricus SegA-ADP complex bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-07
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	220.000, 60.541, 132.143
Unit cell angles	90.00, 127.01, 90.00

Refinement procedure

Resolution	27.680 - 2.800
R-factor	0.2428
Rwork	0.239
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dut
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.850
High resolution limit [Å]	2.800	7.560	2.800
Rmerge	0.107	0.043	0.450
Rmeas	0.127	0.053	0.532
Rpim	0.066	0.031	0.281
Total number of observations	117429
Number of reflections	32772	1667	1595
<I/σ(I)>	6.9
Completeness [%]	94.4	92	92.4
Redundancy	3.6	3	3.4
CC(1/2)		0.995	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MES sodium salt pH 6.5, PEG8000, calcium acetate.