7DWM

Crystal structure of the phage VqmA-DPO complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2019-11-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979106
Spacegroup name	P 31 2 1
Unit cell lengths	133.664, 133.664, 89.514
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.430 - 2.650
R-factor	0.2598
Rwork	0.257
R-free	0.29070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ide
RMSD bond length	0.006
RMSD bond angle	1.113
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	115.760	2.780
High resolution limit [Å]	2.650	2.650
Rmerge	0.068	0.922
Rmeas		0.979
Rpim		0.323
Number of reflections	25381	2882
<I/σ(I)>	18.4	2.3
Completeness [%]	93.4
Redundancy	8.9
CC(1/2)	0.999	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.05 M TRIS hydrochloride pH 7.2, 0.005 M Magnesium sulfate hydrate, 2.4 M 1,6-Hexanediol