7DUV

Structure of Sulfolobus solfataricus SegB protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-02-28
Detector	RAYONIX MX300HE
Wavelength(s)	0.993
Spacegroup name	P 43 21 2
Unit cell lengths	61.773, 61.773, 171.925
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.070 - 3.200
R-factor	0.2849
Rwork	0.280
R-free	0.32570
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.310
High resolution limit [Å]	3.200	6.870	3.200
Rmerge	0.158	0.035	1.120
Rmeas	0.164	0.037	1.171
Rpim	0.046	0.011	0.333
Total number of observations	72822
Number of reflections	5959	670	546
<I/σ(I)>	6.4
Completeness [%]	99.1	97.5	96.5
Redundancy	12.2	10.9	10.5
CC(1/2)		1.000	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	ammonium sulfate, PEG 4000