7DUN

Crystal structure of Fab fragment of Daratumumab

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-23
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9798
Spacegroup name	P 65 2 2
Unit cell lengths	89.905, 89.905, 217.477
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.258 - 1.607
R-factor	0.2056
Rwork	0.205
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cmh
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.640
High resolution limit [Å]	1.600	1.600
Rmerge	0.100
Number of reflections	68212	68212
<I/σ(I)>	30.5
Completeness [%]	100.0
Redundancy	17
CC(1/2)	0.996	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION		291	0.2M Potassium phosphate monobasic, 20% w/v Polyethylene glycol 3350