7DU7

Crystal structure of the rationally designed mkDPBB_sym1 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-29
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1
Spacegroup name	P 31
Unit cell lengths	31.259, 31.259, 132.177
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.071 - 1.201
R-factor	0.2045
Rwork	0.204
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7du6
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.270
High resolution limit [Å]	1.200	1.200
Number of reflections	44946	7142
<I/σ(I)>	16.14
Completeness [%]	99.7
Redundancy	4.88
CC(1/2)	0.999	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2100mM Maleic acid