7DTZ
FGFR4 complex with a covalent inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 85 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-07-04 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5870 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.182, 61.434, 60.504 |
| Unit cell angles | 90.00, 97.65, 90.00 |
Refinement procedure
| Resolution | 42.912 - 2.010 |
| R-factor | 0.1667 |
| Rwork | 0.165 |
| R-free | 0.19760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jpj |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.875 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.912 | 2.082 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.052 | 0.152 |
| Rmeas | 0.057 | |
| Number of reflections | 20468 | 1985 |
| <I/σ(I)> | 26.48 | 9.97 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.999 | 0.982 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 0.1M Bis-Tris, 20% PEG 3350, 0.2M LiSO4, pH 4.5 |
