7DTZ

FGFR4 complex with a covalent inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	85
Detector technology	IMAGE PLATE
Collection date	2019-07-04
Detector	RIGAKU
Wavelength(s)	1.5870
Spacegroup name	P 1 21 1
Unit cell lengths	42.182, 61.434, 60.504
Unit cell angles	90.00, 97.65, 90.00

Refinement procedure

Resolution	42.912 - 2.010
R-factor	0.1667
Rwork	0.165
R-free	0.19760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jpj
RMSD bond length	0.004
RMSD bond angle	0.875
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.912	2.082
High resolution limit [Å]	2.010	2.010
Rmerge	0.052	0.152
Rmeas	0.057
Number of reflections	20468	1985
<I/σ(I)>	26.48	9.97
Completeness [%]	99.8
Redundancy	6.2
CC(1/2)	0.999	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	277	0.1M Bis-Tris, 20% PEG 3350, 0.2M LiSO4, pH 4.5