7DTO
Human Annexin A2 with C132-C261 intramolecular disulfide bond
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | RIGAKU R-AXIS IV |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-03-22 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.050, 49.292, 57.355 |
| Unit cell angles | 90.00, 105.36, 90.00 |
Refinement procedure
| Resolution | 55.310 - 2.800 |
| R-factor | 0.2187 |
| Rwork | 0.218 |
| R-free | 0.22250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hyu |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.310 | 2.873 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.219 | 0.298 |
| Number of reflections | 29312 | 521 |
| <I/σ(I)> | 4.215 | |
| Completeness [%] | 98.7 | |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 289 | 13% PEG 550 MME, 0.1 M Bis-Tris pH 6.5, 50 mM NaCl |
