7DT0
Proline hydroxylase H11-N101I mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 121.910, 138.640, 152.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 102.600 - 2.430 |
| R-factor | 0.2044 |
| Rwork | 0.201 |
| R-free | 0.26980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6LNS |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.856 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.600 | 2.510 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.127 | 0.721 |
| Rmeas | 0.773 | |
| Number of reflections | 97664 | 8710 |
| <I/σ(I)> | 35.44 | |
| Completeness [%] | 99.8 | |
| Redundancy | 13.4 | |
| CC(1/2) | 0.998 | 0.947 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.2 M Potassium sodium tartrate tetrahydrate, 16% w/v Polyethylene glycol 3350 |
