7DS8

Crystal structure of actin capping protein in complex with twinflin-1/CD2AP CPI chimera peptide (CDN-TWC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	90
Detector technology	CCD
Collection date	2020-12-23
Detector	ADSC QUANTUM 270
Wavelength(s)	1.12
Spacegroup name	P 21 21 21
Unit cell lengths	59.690, 63.840, 144.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.530 - 1.950
R-factor	0.189
Rwork	0.187
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ds2
RMSD bond length	0.005
RMSD bond angle	0.731
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.325	48.325	2.070
High resolution limit [Å]	1.950	5.810	1.950
Rmerge	0.107	0.030	0.772
Rmeas	0.116	0.033	0.832
Number of reflections	41266	1697	6572
<I/σ(I)>	15.4	52.74	2.61
Completeness [%]	99.9	99	99.6
Redundancy	7.165	6.461	7.108
CC(1/2)	0.998	0.999	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	4% (w/v) PEG 3350, 50mM Tris-HCl (pH = 7.0)