7DS7
The Crystal Structure of Leaf-branch compost cutinase from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-11-17 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 63 |
| Unit cell lengths | 109.412, 109.412, 35.137 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.380 - 2.150 |
| Rwork | 0.180 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6tht |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.380 | 2.220 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.101 | 0.262 |
| Number of reflections | 13146 | 1117 |
| <I/σ(I)> | 22.1 | |
| Completeness [%] | 98.1 | |
| Redundancy | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.6M imidazole, 0.1M tri-sodium citrate PH7.8 |
