7DS4
Crystal structure of actin capping protein in complex with twinflin-1 C-terminus tail F323K mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AichiSR BEAMLINE BL2S1 |
Synchrotron site | AichiSR |
Beamline | BL2S1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2020-09-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.12 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.450, 66.720, 164.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.220 - 1.850 |
R-factor | 0.2118 |
Rwork | 0.209 |
R-free | 0.25890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ds2 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.843 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.220 | 44.220 | 1.960 |
High resolution limit [Å] | 1.850 | 5.510 | 1.850 |
Rmerge | 0.062 | 0.030 | 0.615 |
Rmeas | 0.068 | 0.033 | 0.668 |
Number of reflections | 50056 | 2061 | 7944 |
<I/σ(I)> | 17.73 | 46.96 | 2.63 |
Completeness [%] | 99.8 | 98.8 | 99.5 |
Redundancy | 6.353 | 5.992 | 6.505 |
CC(1/2) | 0.999 | 0.999 | 0.874 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 5% (w/v) PEG 3350, 5% (v/v) ethanol, 50mM Tris-HCl (pH = 7.0) |