7DS3

Crystal structure of actin capping protein in complex with twinflin-2 C-terminus tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	90
Detector technology	CCD
Collection date	2020-01-16
Detector	ADSC QUANTUM 270
Wavelength(s)	1.12
Spacegroup name	P 21 21 21
Unit cell lengths	51.510, 67.640, 164.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.170 - 2.090
R-factor	0.2168
Rwork	0.215
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ds2
RMSD bond length	0.003
RMSD bond angle	0.541
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.170	49.170	2.220
High resolution limit [Å]	2.090	6.230	2.090
Rmerge	0.058	0.022	0.693
Rmeas	0.062	0.024	0.748
Number of reflections	34883	1440	5526
<I/σ(I)>	22.82	70.6	2.84
Completeness [%]	99.9	98	99.8
Redundancy	7.148	6.235	7.133
CC(1/2)	0.999	0.999	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	5% (w/v) PEG 3350, 50mM Tris-HCl (pH = 7.0)