7DRV
Structural basis of SARS-CoV-2-closely-related bat coronavirus RaTG13 to hACE2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.276, 122.875, 110.742 |
| Unit cell angles | 90.00, 95.07, 90.00 |
Refinement procedure
| Resolution | 48.940 - 3.090 |
| R-factor | 0.2272 |
| Rwork | 0.226 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lzg |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.478 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.940 | 3.210 |
| High resolution limit [Å] | 3.090 | 3.100 |
| Rmerge | 0.106 | 1.310 |
| Number of reflections | 39622 | 3806 |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M succinic acid pH 7.0, 0.1 M bicine pH 8.5, 30% v/v polyethylene glycol monomethyl ether 550 |
