7DRR

Structure of SspE-R100A protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-10
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	110.028, 137.970, 292.354
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.540 - 3.480
R-factor	0.2276
Rwork	0.224
R-free	0.29420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jiv
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.560
High resolution limit [Å]	3.480	3.480
Number of reflections	56714	2809
<I/σ(I)>	2
Completeness [%]	99.9
Redundancy	12.9
CC(1/2)	0.988	0.583

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	287.15	10% PEG 3350, 4% Tacsimate pH 7.0