7DQN

The structure of the Arabidopsis thaliana guanosine deaminase mutant Y185F complexed with guanosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2020-12-16
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 61
Unit cell lengths	119.171, 119.171, 39.476
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.780 - 2.600
R-factor	0.1682
Rwork	0.165
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dbf
RMSD bond length	0.003
RMSD bond angle	0.562
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.780	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.162	0.609
Number of reflections	10100	1442
<I/σ(I)>	12.1	3.7
Completeness [%]	99.9	100
Redundancy	6.9	7.1
CC(1/2)	0.992	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	1.2 M Na-citrate and 0.1 M HEPES (pH 7.5)