7DPQ
HIV-1 Protease D30N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.934000 |
| Spacegroup name | P 61 |
| Unit cell lengths | 62.370, 62.370, 82.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.160 - 1.650 |
| R-factor | 0.1931 |
| Rwork | 0.191 |
| R-free | 0.22540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kt2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.878 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.160 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.044 | 0.581 |
| Number of reflections | 21751 | 3438 |
| <I/σ(I)> | 24.23 | |
| Completeness [%] | 99.5 | |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 1-5% Saturated Ammonium Sulfate, 200 mM /100 mM Phosphate Citrate Buffer pH 6.2 |
