7DPH
H-Ras Q61H in complex with GppNHp (state 1) after structural transition by humidity control
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-01-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 91.769, 91.769, 121.268 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.208 - 1.540 |
Rwork | 0.192 |
R-free | 0.20230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k8y |
RMSD bond length | 0.004 |
RMSD bond angle | 1.293 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.570 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.043 | 0.333 |
Number of reflections | 29269 | 1676 |
<I/σ(I)> | 68.4 | 9.3 |
Completeness [%] | 99.8 | |
Redundancy | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | PEG400, SODIUM ACETATE, CALCIUM ACETATE |