7DON

Structure of tubulin H392D mutant from Odinarchaeota

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	40.210, 92.614, 102.143
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.310 - 1.800
Rwork	0.171
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6o2r
RMSD bond length	0.014
RMSD bond angle	1.286
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.310	1.870
High resolution limit [Å]	1.800	1.800
Rmerge	0.072	1.120
Rpim	0.031	0.464
Number of reflections	35951	3485
<I/σ(I)>	14.2	1.6
Completeness [%]	99.7
Redundancy	6.5
CC(1/2)	0.999	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M sodium acetate trihydrate, 0.1 M Bis-Tris propane, pH 8.5, 25 % w/v PEG 3350