7DOH
Crystal Structure of GD-26 Fab in Complex with TD Peptide from Haloarcula Marismortui Bacteriorhodopsin I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2019-10-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.427, 74.341, 108.103 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.301 - 1.450 |
| R-factor | 0.132549387016 |
| Rwork | 0.130 |
| R-free | 0.17771 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zxb |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.830 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11_2567) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.500 |
| High resolution limit [Å] | 1.446 | 1.450 |
| Number of reflections | 74312 | 7348 |
| <I/σ(I)> | 29 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.970 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 25% (w/v) PEG 3350, 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5 |
