7DNU

mRNA-decapping enzyme g5Rp with inhibitor insp6 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	CCD
Collection date	2019-07-11
Detector	RAYONIX MX300HE
Wavelength(s)	0.9785
Spacegroup name	P 41 2 2
Unit cell lengths	48.432, 48.432, 220.077
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.920 - 2.245
R-factor	0.1739
Rwork	0.171
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dnt
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.290
High resolution limit [Å]	2.245	6.100	2.250
Rmerge	0.067	0.050	0.530
Rmeas	0.069	0.052	0.552
Rpim	0.017	0.014	0.147
Number of reflections	13490	803	668
<I/σ(I)>	19
Completeness [%]	99.9	99.1	99.7
Redundancy	15	13.6	12.2
CC(1/2)		0.999	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289.15	1M imidazole