7DNT

mRNA-decapping enzyme g5Rp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	CCD
Collection date	2019-07-11
Detector	RAYONIX MX300HE
Wavelength(s)	0.9785
Spacegroup name	P 21 21 2
Unit cell lengths	56.083, 105.113, 49.523
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.715 - 2.500
R-factor	0.2029
Rwork	0.194
R-free	0.28510
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.499	6.780	2.500
Rmerge	0.127	0.076	0.305
Rmeas	0.134	0.081	0.346
Rpim	0.041	0.025	0.157
Number of reflections	17165	603	248
<I/σ(I)>	7.9
Completeness [%]	91.7	99.2	48.8
Redundancy	9.1	9.5	3.4
CC(1/2)		0.997	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	289.15	0.1M sodium citrate tribasic dihydrate (pH 5.8), 0.54M magnesium formate dihydrate, 10% (v/v) 1,2-butanediol as an additive reagent