7DNE
DARPin 5m3_D12 in complex with V3-IY (MN) crown mimetic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.330, 51.800, 57.210 |
| Unit cell angles | 78.97, 80.89, 80.42 |
Refinement procedure
| Resolution | 40.950 - 1.900 |
| R-factor | 0.2342 |
| Rwork | 0.232 |
| R-free | 0.26610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SVX chain A |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.951 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.057 | 0.687 |
| Rmeas | 0.069 | |
| Number of reflections | 21900 | 2238 |
| <I/σ(I)> | 10.7 | 1.93 |
| Completeness [%] | 92.2 | 93.07 |
| Redundancy | 3.1 | 3.1 |
| CC(1/2) | 0.998 | 0.759 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 30.0% w/v PEG 4000, 0.2M Li2SO4, 0.02M TAPS pH 9 |
