7DNA

Photocleavable Fluorescent Protein in green and red form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9800
Spacegroup name	P 1
Unit cell lengths	38.930, 72.490, 126.550
Unit cell angles	92.46, 97.31, 92.50

Refinement procedure

Resolution	46.270 - 2.300
R-factor	0.2232
Rwork	0.221
R-free	0.27070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hqk
RMSD bond length	0.004
RMSD bond angle	0.863
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.270	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.042
Number of reflections	58440	9388
<I/σ(I)>	9.6
Completeness [%]	95.9
Redundancy	1.8
CC(1/2)	0.998	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M MIB buffer pH 6.0, 25% w/v PEG 1500