7DN9
Crystal structure of Salmonella effector in complex with NAD and host co-factor ARF1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2016-11-18 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 104.711, 105.576, 105.839 |
Unit cell angles | 90.00, 104.80, 90.00 |
Refinement procedure
Resolution | 46.910 - 3.290 |
R-factor | 0.2388 |
Rwork | 0.236 |
R-free | 0.27680 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.427 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.920 | 3.370 |
High resolution limit [Å] | 3.287 | 3.290 |
Rmerge | 0.156 | 0.501 |
Rmeas | 0.193 | 0.625 |
Number of reflections | 33860 | 2301 |
<I/σ(I)> | 5.79 | 2.03 |
Completeness [%] | 97.1 | 92.6 |
Redundancy | 2.86 | 2.63 |
CC(1/2) | 0.975 | 0.733 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 13% PEG 3350, 300mM sodium citrate, 100mM Bis-Tris pH 6.5 |