7DMM
Structure of a glucose isomerase crystal grown in an aqueous glycerol solution without any precipitants
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2019-06-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.610, 98.460, 102.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.160 - 0.990 |
| R-factor | 0.15736 |
| Rwork | 0.157 |
| R-free | 0.16564 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xib |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.290 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.940 | 1.010 |
| High resolution limit [Å] | 0.990 | 0.990 |
| Rmerge | 0.046 | 0.308 |
| Number of reflections | 256074 | 12629 |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.4 | |
| CC(1/2) | 0.998 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | precipitant-free in 30(v/v)% aqueous glycerol solution |
