7DLZ

Crystal Structure of Methyltransferase Ribozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-12-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	62.784, 80.176, 103.296
Unit cell angles	90.00, 91.71, 90.00

Refinement procedure

Resolution	29.776 - 3.002
R-factor	0.2147
Rwork	0.212
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1urn
RMSD bond length	0.006
RMSD bond angle	0.937
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.110
High resolution limit [Å]	3.000	6.440	3.000
Rmerge	0.071	0.045	0.343
Rmeas	0.079	0.049	0.413
Rpim	0.034	0.019	0.223
Total number of observations	84155
Number of reflections	19768	2122	1834
<I/σ(I)>	8
Completeness [%]	96.0	99.9	89.2
Redundancy	4.3	6	2.6
CC(1/2)		0.998	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	polyethylene glycol 3350