7DLZ
Crystal Structure of Methyltransferase Ribozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-12-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.784, 80.176, 103.296 |
| Unit cell angles | 90.00, 91.71, 90.00 |
Refinement procedure
| Resolution | 29.776 - 3.002 |
| R-factor | 0.2147 |
| Rwork | 0.212 |
| R-free | 0.26630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1urn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.937 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.440 | 3.000 |
| Rmerge | 0.071 | 0.045 | 0.343 |
| Rmeas | 0.079 | 0.049 | 0.413 |
| Rpim | 0.034 | 0.019 | 0.223 |
| Total number of observations | 84155 | ||
| Number of reflections | 19768 | 2122 | 1834 |
| <I/σ(I)> | 8 | ||
| Completeness [%] | 96.0 | 99.9 | 89.2 |
| Redundancy | 4.3 | 6 | 2.6 |
| CC(1/2) | 0.998 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | polyethylene glycol 3350 |
