7DLW

Crystal structure of Arabidopsis ACS7 in complex with PPG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	66.877, 69.353, 85.400
Unit cell angles	97.70, 94.97, 106.50

Refinement procedure

Resolution	45.200 - 2.190
R-factor	0.1945
Rwork	0.193
R-free	0.24080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3piu
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.260
High resolution limit [Å]	2.190	2.200
Rmerge	0.085	0.474
Number of reflections	71190	5851
<I/σ(I)>	10.5
Completeness [%]	96.2
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M Tris pH 8.6, 23% (w/v) PEG 3350