7DL1

The mutant E310G/G323S structure of 3,5-DAHDHcca complex with NADPH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-20
Detector	PSI PILATUS 6M
Wavelength(s)	0.97930
Spacegroup name	C 1 2 1
Unit cell lengths	106.241, 127.874, 116.994
Unit cell angles	90.00, 96.30, 90.00

Refinement procedure

Resolution	26.736 - 2.720
R-factor	0.194548456194
Rwork	0.192
R-free	0.23878
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.939
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.770
High resolution limit [Å]	2.715	2.720
Rmerge	0.165	1.121
Number of reflections	41956	2097
<I/σ(I)>	14.182
Completeness [%]	100.0
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	1.0 M LiCl, 0.1 M Tris-HCl buffer (pH 7.0), 11% w/v PEG 6000