7DL0

The mutant E310G/A314Y of 3,5-DAHDHcca complex with NADPH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-11
Detector	PSI PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	I 41 2 2
Unit cell lengths	161.882, 161.882, 125.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.560 - 2.170
R-factor	0.2143
Rwork	0.211
R-free	0.26850
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.210
High resolution limit [Å]	2.170	2.170
Rmerge	0.270	1.542
Rpim		0.501
Number of reflections	44317	2029
<I/σ(I)>	16.3
Completeness [%]	99.2
Redundancy	0.27

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M MgSO4, 14% w/v PEG 3000