7DKL
Crystal structure of the tandem DEP domains of DEPTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-26 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.582, 63.582, 89.081 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.876 - 1.500 |
| R-factor | 0.1798 |
| Rwork | 0.178 |
| R-free | 0.21750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o7f |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.777 |
| Data reduction software | DENZO |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
| Rmerge | 0.077 | 0.048 | 0.658 |
| Rmeas | 0.080 | 0.051 | 0.694 |
| Rpim | 0.023 | 0.014 | 0.216 |
| Total number of observations | 412973 | ||
| Number of reflections | 36176 | 2011 | 1758 |
| <I/σ(I)> | 7 | ||
| Completeness [%] | 98.2 | 99.1 | 96.1 |
| Redundancy | 11.4 | 11.3 | 9.6 |
| CC(1/2) | 0.998 | 0.854 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M Ammonium acetate, 24% PEG 3350 |
