7DKL

Crystal structure of the tandem DEP domains of DEPTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-26
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	39.582, 63.582, 89.081
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.876 - 1.500
R-factor	0.1798
Rwork	0.178
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o7f
RMSD bond length	0.005
RMSD bond angle	0.777
Data reduction software	DENZO
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.077	0.048	0.658
Rmeas	0.080	0.051	0.694
Rpim	0.023	0.014	0.216
Total number of observations	412973
Number of reflections	36176	2011	1758
<I/σ(I)>	7
Completeness [%]	98.2	99.1	96.1
Redundancy	11.4	11.3	9.6
CC(1/2)		0.998	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M Ammonium acetate, 24% PEG 3350