7DK9
Crystal structure of DsbA-like protein DR2335 from Deinococcus radiodurans R1, native protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-25 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.475, 87.389, 63.414 |
Unit cell angles | 90.00, 90.09, 90.00 |
Refinement procedure
Resolution | 31.510 - 1.720 |
R-factor | 0.1793 |
Rwork | 0.178 |
R-free | 0.22010 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.750 |
High resolution limit [Å] | 1.720 | 1.720 |
Rmerge | 0.056 | 0.182 |
Rmeas | 0.061 | 0.204 |
Rpim | 0.024 | 0.089 |
Number of reflections | 51343 | 2628 |
<I/σ(I)> | 38.8 | 7.932 |
Completeness [%] | 97.2 | |
Redundancy | 5.7 | |
CC(1/2) | 0.954 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 295 | Sodium potassium phosphate pH 7.0, 8% w/v PEG 500 MME |