7DJQ
Crystal Structure of O-acetyl L-serine sulfhydrylase from Haemophilus influenzae in complex with C-Terminal peptide of ribosomal S4 Domain protein from Lactobacillus salivarius.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-03-19 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.080, 98.940, 70.290 |
| Unit cell angles | 90.00, 95.77, 90.00 |
Refinement procedure
| Resolution | 40.860 - 2.300 |
| R-factor | 0.1912 |
| Rwork | 0.189 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ho1 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.516 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.860 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.063 | 0.150 |
| Number of reflections | 28307 | 2705 |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 97.7 | 94.98 |
| Redundancy | 15.2 | 9 |
| CC(1/2) | 0.855 | 0.826 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 100mM HEPES buffer pH 7.4, 1.4M Sodium Citrate |
