7DJK

Structure of four truncated and mutated forms of quenching protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-03-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	I 4 2 2
Unit cell lengths	183.191, 183.191, 120.894
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.840 - 2.801
R-factor	0.179747306216
Rwork	0.178
R-free	0.21847
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gc1
RMSD bond length	0.011
RMSD bond angle	1.223
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rpim	0.050
Number of reflections	25154	1241
<I/σ(I)>	12.13
Completeness [%]	98.5
Redundancy	5.2
CC(1/2)		0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291.15	0.1 M NaCl, 0.1 M tri-Sodium citrate pH 5.6, 12% (w/v) polyethylene glycol 4000 (PEG 4000)