7DI8

Electron crystallographic structure of Catalase using a direct electron detector at 300 kV

Experimental procedure

Collection date	2020-08-26
Wavelength(s)	0.0197
Spacegroup name	P 21 21 21
Unit cell lengths	69.980, 174.020, 199.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	131.126 - 3.200
R-factor	0.3109
Rwork	0.309
R-free	0.34820
RMSD bond length	0.004
RMSD bond angle	0.731
Data scaling software	XSCALE
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	131.126	131.126	3.180
High resolution limit [Å]	3.000	9.000	3.000
Rmerge	1.159	0.557	1.786
Rmeas	1.173	0.566	1.808
Total number of observations	1783075
Number of reflections	40941	1641	6529
<I/σ(I)>	3.79	7.54	1.9
Completeness [%]	82.3	80.8	82.7
Redundancy	43.552	40.983	42.194
CC(1/2)	0.688	0.941	0.185

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	5.3