7DHW

Crystal structure of myosin-XI motor domain in complex with ADP-ALF4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	58.277, 73.725, 187.957
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.810 - 2.840
R-factor	0.2309
Rwork	0.226
R-free	0.27060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mnd
RMSD bond length	0.002
RMSD bond angle	0.490
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.810	19.810	3.010
High resolution limit [Å]	2.840	7.900	2.840
Rmerge	0.132	0.028	0.840
Rmeas	0.147	0.032	0.927
Number of reflections	19625	951	2992
<I/σ(I)>	11.09	40.75	2.1
Completeness [%]	99.1	92.3
Redundancy	5.58
CC(1/2)	0.997	0.999	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	0.1 M MMT buffer (pH 6.0), 25% PEG-1500