7DHD

Vibrio vulnificus Wzb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9788
Spacegroup name	P 21 21 21
Unit cell lengths	47.315, 48.741, 117.316
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.660 - 1.710
R-factor	0.2
Rwork	0.199
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wmy
RMSD bond length	0.010
RMSD bond angle	1.020
Data reduction software	XDS (Jan 31, 2020 (BUILT 20200417))
Data scaling software	Aimless (0.5.29)
Phasing software	PHASER (2.7.17)
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.660	1.740
High resolution limit [Å]	1.710	1.711
Rmeas	0.088	1.238
Rpim	0.025	0.343
Number of reflections	29957	1506
<I/σ(I)>	19.2	2.3
Completeness [%]	99.5	99.7
Redundancy	12.6	12.9
CC(1/2)	0.999	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Free VvWzb crystals were grown in drops containing 1.5 ul of protein solution (14 mg/ml in the buffer 10 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT) and 1.5 ul of reservoir solution (100 mM HEPES pH7.5, 200 mM NaCl, 31% (w/v) PEG 3350)