7DH1

The structure of the Arabidopsis thaliana guanosine deaminase in reaction with N2-Methylguanosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 61
Unit cell lengths	118.776, 118.776, 39.540
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.350 - 1.850
R-factor	0.165
Rwork	0.163
R-free	0.19870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dbf
RMSD bond length	0.014
RMSD bond angle	1.252
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.120	0.739
Number of reflections	27608	2600
<I/σ(I)>	28	2.25
Completeness [%]	99.6	95.6
Redundancy	19.3	16.3
CC(1/2)	0.994	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	1.2M Na-citrate and 0.1M HEPES (pH 7.5)