7DG7
DPBB domain of VCP-like ATPase from Methanopyrus kandleri
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-21 |
Detector | DECTRIS PILATUS3 S 2M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.078, 29.787, 42.155 |
Unit cell angles | 90.00, 106.42, 90.00 |
Refinement procedure
Resolution | 40.436 - 1.604 |
R-factor | 0.2086 |
Rwork | 0.206 |
R-free | 0.22730 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 10094 | 1569 |
<I/σ(I)> | 22.97 | |
Completeness [%] | 98.7 | |
Redundancy | 13.2 | |
CC(1/2) | 0.990 | 0.979 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM imidazole/HCl pH7.8, 2M NaCl, 200mM Zinc acetate |