7DFY
Novel motif for left-handed G-quadruplex formation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.405, 31.601, 77.764 |
| Unit cell angles | 90.00, 98.07, 90.00 |
Refinement procedure
| Resolution | 76.990 - 1.690 |
| R-factor | 0.2039 |
| Rwork | 0.201 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gz6 |
| Data scaling software | XDS |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.995 | 1.904 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.189 | 1.212 |
| Rmeas | 0.206 | |
| Rpim | 0.080 | 0.739 |
| Number of reflections | 9010 | 450 |
| <I/σ(I)> | 8 | 1 |
| Completeness [%] | 86.2 | 40.5 |
| Redundancy | 6.3 | 3.5 |
| CC(1/2) | 0.996 | 0.349 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.08 M Strontium Chloride hexahydrate, 0.04 M Sodium cacodylate trihydrate ph=6.0, 35% v/v (+/-)-2-Methyl-2,4-pentanediol (MPD), 0.012 M Spermine tetrahydrochoride |
