7DFU

Crystal structure of Xanthomonas oryzae ClpP S68Y in complex with ADEP4.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9735
Spacegroup name	P 42 21 2
Unit cell lengths	132.498, 132.498, 187.833
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.692 - 1.901
R-factor	0.2187
Rwork	0.217
R-free	0.24340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sta
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (V1.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.135	0.057	0.494
Rmeas	0.140	0.059	0.556
Rpim	0.032	0.012	0.243
Total number of observations	2033446
Number of reflections	130286	13758	12105
<I/σ(I)>	5.2
Completeness [%]	99.3	99.9	93.6
Redundancy	15.6	22.9	4.3
CC(1/2)		0.999	0.224

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M Sodium acetate trihydrate, 3.0 M Sodium chloride