7DF9
Crystal of Arrestin2-V2Rpp-1-Fab30 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.97736 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 116.174, 121.536, 144.639 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.250 - 3.170 |
R-factor | 0.2566 |
Rwork | 0.255 |
R-free | 0.28050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jqi |
RMSD bond length | 0.012 |
RMSD bond angle | 1.368 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.520 | 3.390 |
High resolution limit [Å] | 3.170 | 3.170 |
Rmerge | 1.459 | |
Rpim | 0.875 | |
Number of reflections | 17687 | 3145 |
<I/σ(I)> | 9.8 | |
Completeness [%] | 99.8 | |
Redundancy | 5.6 | |
CC(1/2) | 0.990 | 0.783 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 289 | 10% PEG 3350, 0.1M Succinic acid, pH 6.5-7.5, 0.2mM DMSO |