7DE9
crystal structure of Arabidopsis RDM15 tudor domain in complex with an H3K4me1 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 28.579, 36.963, 46.678 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.978 - 1.711 |
R-factor | 0.1843 |
Rwork | 0.183 |
R-free | 0.20220 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.954 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
Rmerge | 0.099 | 0.069 | 0.500 |
Rmeas | 0.106 | 0.074 | 0.533 |
Rpim | 0.036 | 0.026 | 0.182 |
Number of reflections | 5630 | 629 | 545 |
<I/σ(I)> | 5.9 | ||
Completeness [%] | 99.9 | 99.8 | 99.8 |
Redundancy | 8.7 | 8.1 | 9 |
CC(1/2) | 0.992 | 0.841 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-HCl, pH 8.5, and 20% PEG 1000 |