7DE8
Crystal Structure of outer membrane protein PorB with G103K mutations from Neisseria meningitidis W135
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 63 |
Unit cell lengths | 84.396, 84.396, 107.110 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.237 - 2.760 |
Rwork | 0.213 |
R-free | 0.26030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vy8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.466 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.300 | 2.810 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.077 | 0.840 |
Number of reflections | 11223 | 792 |
<I/σ(I)> | 22.6 | |
Completeness [%] | 99.8 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM MES, pH 6.5 31% Jeffamine M-600 |