7DE4
Hybrid cluster protein (HCP) from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 142.540, 142.540, 117.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.290 - 3.610 |
R-factor | 0.207 |
Rwork | 0.203 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w9m |
RMSD bond length | 0.011 |
RMSD bond angle | 3.378 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.290 | 3.710 |
High resolution limit [Å] | 3.610 | 3.610 |
Rmerge | 0.159 | 0.975 |
Number of reflections | 14324 | 13606 |
<I/σ(I)> | 14.16 | 3.01 |
Completeness [%] | 99.5 | 99.7 |
Redundancy | 11.48 | 11.98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 2.0M Sodium formate, 0.1M Sodium acetate |