7DDU
Elephant seal myoglobin esMb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-07-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.090, 29.350, 75.360 |
| Unit cell angles | 90.00, 96.20, 90.00 |
Refinement procedure
| Resolution | 19.820 - 1.900 |
| R-factor | 0.1993 |
| Rwork | 0.199 |
| R-free | 0.20490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yce |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.161 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.17rc5_3630) |
| Refinement software | PHENIX (1.17rc5_3630) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.024 | 0.104 |
| Number of reflections | 22841 | 3287 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 99.8 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 3.1M Ammonium sulfate, 5.5% (w/v) PEG 3350 |
