Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 114.973, 118.493, 495.137 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.990 - 3.460 |
R-factor | 0.2425 |
Rwork | 0.241 |
R-free | 0.27180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6KPU |
RMSD bond length | 0.005 |
RMSD bond angle | 0.860 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.550 |
High resolution limit [Å] | 3.450 | 3.450 |
Rmerge | 0.118 | 0.337 |
Number of reflections | 49808 | 179 |
<I/σ(I)> | 8.7 | |
Completeness [%] | 55.7 | |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | 16% PEG 2000 MME, 10% GLYCEROL, 200mM MAGNESIUM CHLORIDE, 5mM GSH, 0.1mM DTT, 0.0001% BHT, 100mM MES-NMDG, PH 6.2 |