7DDA

Envelope protein VP37 a crystal structure from White Spot Syndrome Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9800
Spacegroup name	P 63 2 2
Unit cell lengths	89.382, 89.382, 99.046
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.740 - 2.510
R-factor	0.2047
Rwork	0.201
R-free	0.23800
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.912
Data reduction software	XDS (Jan 31, 2020)
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX (1.18.2-3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.691	2.548
High resolution limit [Å]	2.505	2.505
Rmerge	0.085	0.327
Rmeas	0.086	0.331
Rpim	0.014	0.054
Number of reflections	8198	410
<I/σ(I)>	43.6	12.2
Completeness [%]	96.2	100
Redundancy	37.1	36.1
CC(1/2)	1.000	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.2	1.5 M Ammonium sulfate, 100 mM Bis-Tris pH 6.5, 100 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293.2K