7DD0
Crystal structure of the N-terminal domain of TagH from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-24 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.735, 90.667, 91.213 |
| Unit cell angles | 90.00, 91.77, 90.00 |
Refinement procedure
| Resolution | 19.730 - 2.700 |
| R-factor | 0.1886 |
| Rwork | 0.186 |
| R-free | 0.23490 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.771 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.735 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.123 | 0.495 |
| Number of reflections | 32572 | 3161 |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 98.8 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% polyethylene glycol (PEG) 3,000 and 0.1 M sodium citrate (pH 5.5) |
