7DCY

Apo form of Mycoplasma genitalium RNase R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	69.652, 80.768, 176.476
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.550 - 1.972
R-factor	0.1747
Rwork	0.173
R-free	0.19980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xgu
RMSD bond length	0.011
RMSD bond angle	0.978
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2148)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.010
High resolution limit [Å]	1.970	1.970
Rmerge	0.175	2.931
Number of reflections	70690	3444
<I/σ(I)>	18.6	1.1
Completeness [%]	99.9
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	100 mM KCl, 100 mM HEPES (pH 6.8), 15% PEG 5000MME