7DCI
Crystal structure of HSF2 DNA-binding domain in complex with 2-site HSE DNA in the head-to-head orientation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-06-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.268, 38.472, 38.654 |
| Unit cell angles | 90.00, 100.74, 90.00 |
Refinement procedure
| Resolution | 37.977 - 1.700 |
| R-factor | 0.1806 |
| Rwork | 0.179 |
| R-free | 0.20940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hdk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.740 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.980 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rpim | 0.034 | 0.150 |
| Number of reflections | 13957 | 13957 |
| <I/σ(I)> | 25.4 | |
| Completeness [%] | 94.0 | |
| Redundancy | 7.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M lithium citrate and 23% PEG 3350 |
